e-Science : Oct 2016 Issue 18
STAYIN’ ALIVE RESOURCES A purpose built chemostat that allows continuous bacterial growth for prolonged time periods. This chemostat was kindly provided and run in collaboration with Dr Peter Zilm from the School of Dentistry at the University of Adelaide. there is a central, common molecular process). It is also extremely difficult to capture the cells in this state to study the process of switching to a SCV form. So the question has been how do we accurately study bacteria that exist in a dormant or quasi-dormant state? The research we have undertaken here at the University of Adelaide has used a broad collection of S. aureus samples from different hospitals. These are from a range of sources including blood, skin, CRS, osteomyelitis and soft tissue infection (SSTI), and upper and lower respiratory tract infections. From these strains, we identified those strains that are predisposed to SCV formation and identified differences in the frequency of SCV formation. This data shows that some S. aureus clinical isolates are capable of forming the dormant state SCV cells while others are not. It also shows that hostgenerated chemical stresses and antibiotics can induce a switch to the SCV state.
May 2016 Issue 17